Department of Eukaryote Gene Engineering

Main publications

2024

1. Kalvaitytė M, Gabrilavičiūtė S, Balciunas D. Rapid generation of single-insertion transgenics by Tol2 transposition in zebrafish. Dev Dyn. 2024 Nov;253(11):1056-1065. doi: 10.1002/dvdy.719. Epub 2024 Jun 30.PMID: 38946125
2. Vasiliūnaitė E, Repšytė M, Kramer EM, Lang J, Jelinek C, Ulrich RG, Buck CB, Gedvilaitė A. Novel polyomavirus in the endangered garden dormouse Eliomys quercinus. A.Virol J. 2024 Nov 27;21(1):309. doi: 10.1186/s12985-024-02581-x.PMID: 39605065
3. Ziogiene D, Burdulis A, Timinskas A, Zinkeviciute R, Vasiliunaite E, Norkienė M, Gedvilaite A. Dolichol kinases from yeast, nematode and human can replace each other and exchange their domains creating active chimeric enzymes in yeast. 2024 Nov 7, PLoS ONE 19(11): e0313330. doi.org/10.1371/journal.pone.0313330

2023

1. Sližienė A, Plečkaitytė M, Rudokas V, Juškaitė K, Žvirblis G, Žvirblienė A. (2023). Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts. Molecular Immunology. doi: 10.1016/j.molimm.2023.01.001

2022

1. Jiang Z, El-Brolosy MA, Serobyan V, Welker JM, Retzer N, Dooley CM, Jakutis G, Juan T, Fukuda N, Maischein HM, Balciunas D, Stainier DYR. (2022). Parental mutations influence wild-type offspring via transcriptional adaptation. Science Advances. doi: 10.1126/sciadv.abj2029.
2. Kalvaityte M, Balčiūnas D. (2022). Conditional mutagenesis strategies in zebrafish. Trends in Genetics. https://doi.org/10.1016/j.tig.2022.04.007.
3. Zinkevičiūtė R, Ražanskas R, Kaupinis A, Macijauskaitė N, Čiplys E, Houen G, Slibinskas R. (2022). Yeast Secretes High Amounts of Human Calreticulin without Cellular Stress. Curr Issues Mol Biol. 2022 Apr 19;44(5):1768-1787. doi: 10.3390/cimb44050122.
4. Mughal FP, Bergmann AC, Huynh HUB, Jørgensen SH, Mansha I, Kesmez M, Schürch PM, Theocharides APA, Hansen PR, Friis T, Holmström MO, Ciplys E, Slibinskas R, Højrup P, Houen G, Trier NH. (2022). Characterization of Peptide Antibodies to the C-Terminal of Frameshifted Calreticulin Associated with Myeloproliferative Diseases. doi: 10.3390/ijms23126803.
5. Drobysh M, Liustrovaite V, Baradoke A, Rucinskiene A, Ramanaviciene A, Ratautaite V, Viter R, Chen CF, Plikusiene I, Samukaite-Bubniene U, Slibinskas R, Ciplys E, Simanavicius M, Zvirbliene A, Kucinskaite-Kodze I, Ramanavicius A. (2022) Electrochemical Determination of Interaction between SARS-CoV-2 Spike Protein and Specific Antibodies. Int J Mol Sci. doi: 10.3390/ijms23126768.
6. Kyllesbech C, Trier N, Slibinskas R, Čiplys E, Tsakiri A, Frederiksen JL, Houen G. (2022). Virus-specific antibody indices may supplement the total IgG index in diagnostics of multiple sclerosis. J Neuroimmunol. doi: 10.1016/j.jneuroim.2022.577868.
7. Ratautaite V, Boguzaite R, Brazys E, Ramanaviciene A, Čiplys E, Juozapaitis M, Slibinskas R, Bechelany M, Ramanavicius A. (2022). Molecularly imprinted polypyrrole based sensor for the detection of SARS-CoV-2 spike glycoprotein. Electrochim Acta. doi: 10.1016/j.electacta.2021.139581.
8. Trier NH, Valdarnini N, Fanelli I, Rovero P, Hansen PR, Schafer-Nielsen C, Čiplys E, Slibinskas R, Pociot F, Friis T, Houen G. (2022). Peptide Antibody Reactivity to Homologous Regions in Glutamate Decarboxylase Isoforms and Coxsackievirus B4 P2C. Int J Mol Sci. doi: 10.3390/ijms23084424.
9. Paškevičius Š, Dapkutė V, Misiūnas A, Balzaris M, Thommes P, Sattar A, Gleba Y, Ražanskienė A. (2022). Chimeric bacteriocin S5-PmnH engineered by domain swapping efficiently controls Pseudomonas aeruginosa infection in murine keratitis and lung models. Sci Rep. doi: 10.1038/s41598-022-09865-8.
10. Paškevičius Š, Gleba Y, Ražanskienė A. (2022). Stenocins: Novel modular bacteriocins from opportunistic pathogen Stenotrophomonas maltophilia. J Biotechnol. doi: 10.1016/j.jbiotec.2022.04.006.
11. Lučiūnaitė A, Dalgėdienė I, Žilionis R, Mašalaitė K, Norkienė M, Šinkūnas A, Gedvilaitė A, Kučinskaitė-Kodzė I, Žvirblienė A. (2022). Activation of NLRP3 Inflammasome by Virus-Like Particles of Human Polyomaviruses in Macrophages. Frontiers in Immunology. doi: 10.3389/fimmu.2022.831815.

2021

1. Jandrig B, Krause H, Zimmermann W, Vasiliunaite E, Gedvilaite A, Ulrich RG. (2021). Hamster Polyomavirus Research: Past, Present, and Future. Viruses 13(5):907. doi: 10.3390/v13050907.
2. Eiden M, Gedvilaite A, Leidel F, Ulrich RG, Groschup MH. (2021). Vaccination with Prion Peptide-Displaying Polyomavirus-Like Particles Prolongs Incubation Time in Scrapie-Infected Mice. Viruses 13(5):811. doi: 10.3390/v13050811.
3. Houen G, Højrup P, Ciplys E, Gaboriaud C, Slibinskas R. (2021). Structural Analysis of Calreticulin, an Endoplasmic Reticulum-Resident Molecular Chaperone. Prog Mol Subcell Biol. 2021;59:13-25. doi: 10.1007/978-3-030-67696-4_2.
4. Plikusiene I, Maciulis V, Ramanaviciene A, Balevicius Z, Buzavaite-Verteliene E, Ciplys E, Slibinskas R, Simanavicius M, Zvirbliene A, Ramanavicius A.J. (2021). Evaluation of kinetics and thermodynamics of interaction between immobilized SARS-CoV-2 nucleoprotein and specific antibodies by total internal reflection ellipsometry. Colloid Interface Sci. 2021 Jul 15;594:195-203. doi: 10.1016/j.jcis.2021.02.100. Epub 2021 Mar 10.
5. Bulavaitė A, Maier T, Pleckaityte M. (2021) Discrimination of Gardnerella Species by Combining MALDI-TOF Protein Profile, Chaperonin cpn60 Sequences, and Phenotypic Characteristics. Pathogens. 2021 Mar 1;10(3):277. doi: 10.3390/pathogens10030277. PMID: 33804525; PMCID: PMC7998583.
6. Čiplys E, Paškevičius T, Žitkus E, Bielskis J, Ražanskas R, Šneideris T, Smirnovas V, Kaupinis A, Tester DJ, Ackerman MJ, Højrup P, Michalak M, Houen G, Slibinskas R. (2021). Mapping human calreticulin regions important for structural stability. Biochim Biophys Acta Proteins Proteom. 1869(11):140710. doi: 10.1016/j.bbapap.2021.140710.
7. McNabb L, Andiani A, Bulavaite A, Zvirbliene A, Sasnauskas K, Lunt R. (2021). Development and validation of an IgM antibody capture ELISA for early detection of Hendra virus. J Virol Methods. 21;298:114296. doi:10.1016/j.jviromet.2021.114296.
8. Dudas G, Hong SL, Potter BI, Calvignac-Spencer S, Niatou-Singa FS, Tombolomako TB, Fuh-Neba T, Vickos U, Ulrich M, Leendertz FH, Khan K, Huber C, Watts A, Olendraitė I, Snijder J, Wijnant KN, Bonvin AMJJ, Martres P, Behillil S, Ayouba A, Maidadi MF, Djomsi DM, Godwe C, Butel C, Šimaitis A, Gabrielaitė M, Katėnaitė M, Norvilas R, Raugaitė L, Koyaweda GW, Kandou JK, Jonikas R, Nasvytienė I, Žemeckienė Ž, Gečys D, Tamušauskaitė K, Norkienė M, Vasiliūnaitė E, Žiogienė D, Timinskas A, Šukys M, Šarauskas M, Alzbutas G, Aziza AA, Lusamaki EK, Cigolo JM, Mawete FM, Lofiko EL, Kingebeni PM, Tamfum JM, Belizaire MRD, Essomba RG, Assoumou MCO, Mboringong AB, Dieng AB, Juozapaitė D, Hosch S, Obama J, Ayekaba MO, Naumovas D, Pautienius A, Rafaï CD, Vitkauskienė A, Ugenskienė R, Gedvilaitė A, Čereškevičius D, Lesauskaitė V, Žemaitis L, Griškevičius L, Baele G. (2021). Emergence and spread of SARS-CoV-2 lineage B.1.620 with variant of concern-like mutations and deletions. Nat Commun. 2021 Oct 1;12(1):5769. doi: 10.1038/s41467-021-26055-8.
9. Bergmann AC, Kyllesbech C, Slibinskas R, Ciplys E, Højrup P, Trier NH, Houen G. (2021). Epitope Mapping of Monoclonal Antibodies to Calreticulin Reveals That Charged Amino Acids Are Essential for Antibody Binding. Antibodies (Basel). 2021 Aug 4;10(3):31. doi: 10.3390/antib10030031.
10. Lazutka J, Simutis K, Matulis P, Petraitytė-Burneikienė R, Kučinskaitė-Kodzė I, Simanavičius M, Tamošiunas PL. (2021) Antigenicity study of the yeast-generated human parvovirus 4 (PARV4) virus-like particles. Virus Res. 2021 Jan 15;292:198236. doi: 10.1016/j.virusres.2020.198236.
11. Kaliniene L, Noreika A, Kaupinis A, Valius M, Jurgelaitis E, Lazutka J, Meškienė R, Meškys R. (2021). Analysis of a Novel Bacteriophage vB_AchrS_AchV4 Highlights the Diversity of Achromobacter Viruses. Viruses. 2021 Feb 27;13(3):374. doi: 10.3390/v13030374.
12. Kubickova B, Schenk JA, Ramm F, Markuškienė K, Reetz J, Dremsek P, Tamosiunas PL, Cepulyte L, Trinh HA, Scholz J, Memczak H, Hovestädt M, Ryll R, Petraityte-Burneikiene R, Corman VM, Andersson A, Becher D, Groschup MH, Kubick S, Sellrie F, Johne R, Ulrich RG. (2021). A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen. Appl Microbiol Biotechnol. 2021 Jun;105(12):4957-4973. doi: 10.1007/s00253-021-11342-7.
13. Vilys L, Peciuliene I, Jakubauskiene E, Zinkeviciute R, Makino Y, Kanopka A. (2021). U2AF - Hypoxia-induced fas alternative splicing regulator. Exp Cell Res. 2021 Feb 1;399(1):112444. doi: 10.1016/j.yexcr.2020.112444.

2020

1. Starkevič, P., Ražanskienė, A., Starkevič, U., Kazanavičiūtė, V., Denkovskienė, E., Bendokas, V., Šikšnianas, T., Rugienius, R., Stanys, V., and Ražanskas, R. (2020). Isolation and Analysis of Anthocyanin Pathway Genes from Ribes Genus Reveals MYB Gene with Potent Anthocyanin-Inducing Capabilities. Plants 9, 1078. 10.3390/plants9091078
2. Noreika, A., Meškys, R., Lazutka, J., and Kaliniene, L. (2020). Complete genome sequence of Buttiauxella phage vB_ButM_GuL6. Arch Virol 165, 2685-2687. 10.1007/s00705-020-04780-7
3. Lazutka, J., Simutis, K., Matulis, P., Petraitytė-Burneikienė, R., Kučinskaitė-Kodzė, I., Simanavičius, M., and Tamošiunas, P.L. (2020). Antigenicity study of the yeast-generated human parvovirus 4 (PARV4) virus-like particles. Virus Research 198236. 10.1016/j.virusres.2020.198236
4. Špakova, A., Dalgėdienė, I., Insodaitė, R., Sasnauskienė, A., Žvirblienė, A., and Petraitytė-Burneikienė, R. (2020). vB_EcoS_NBD2 bacteriophage-originated polytubes as a carrier for the presentation of foreign sequences. Virus Research 290, 198194. 10.1016/j.virusres.2020.198194
5. Plikusiene, I., Balevicius, Z., Ramanaviciene, A., Talbot, J., Mickiene, G., Balevicius, S., Stirke, A., Tereshchenko, A., Tamosaitis, L., Zvirblis, G., et al. (2020). Evaluation of affinity sensor response kinetics towards dimeric ligands linked with spacers of different rigidity: Immobilized recombinant granulocyte colony-stimulating factor based synthetic receptor binding with genetically engineered dimeric analyte derivatives. Biosensors and Bioelectronics 156, 112112. 1016/j.bios.2020.112112
6. Denkovskienė, E*., Paškevičius, Š*., Stankevičiūtė, J., Gleba, Y., and Ražanskienė, A. (2020). Control of T-DNA Transfer from Agrobacterium tumefaciens to Plants Based on an Inducible Bacterial Toxin-Antitoxin System. MPMI MPMI-03-20-0067. 10.1094/MPMI-03-20-0067-R
7. Vilmane, A., Terentjeva, A., Tamosiunas, P.L., Suna, N., Suna, I., Petraityte-Burneikiene, R., Murovska, M., Rasa-Dzelzkaleja, S., and Nora-Krukle, Z. (2020). Human Parvoviruses May Affect the Development and Clinical Course of Meningitis and Meningoencephalitis. Brain Sciences 10, 339. 10.3390/brainsci10060339
8. Houen, G., Heiden, J., Trier, N.H., Draborg, A.H., Benros, M.E., Zinkevičiūtė, R., Petraitytė-Burneikienė, R., Ciplys, E., Slibinskas, R., and Frederiksen, J.L. (2020). Antibodies to Epstein-Barr virus and neurotropic viruses in multiple sclerosis and optic neuritis. Journal of Neuroimmunology 346, 577314. 10.1016/j.jneuroim.2020.577314
9. Drewes, S., Jeske, K., Straková, P., Balčiauskas, L., Ryll, R., Balčiauskienė, L., Kohlhause, D., Schnidrig, G.-A., Hiltbrunner, M., Špakova, A., Insodaitė R, Petraitytė-Burneikienė R, et al. (2020). Identification of a novel hantavirus strain in the root vole (Microtus oeconomus) in Lithuania, Eastern Europe. Infection, Genetics and Evolution 104520. 10.1016/j.meegid.2020.104520
10. Bulavaitė, A., Dalgediene, I., Michailoviene, V., and Pleckaityte, M. (2020). Type II Restriction-Modification System from Gardnerella vaginalis ATCC 14018. Pathogens 9, 703. 10.3390/pathogens9090703
11. Mickiene, G*., Dalgėdienė, I., Zvirblis, G., Dapkunas, Z., Plikusiene, I., Buzavaite-Verteliene, E., Balevičius, Z., Rukšėnaitė, A., and Pleckaityte, M. (2020). Human granulocyte-colony stimulating factor (G-CSF)/stem cell factor (SCF) fusion proteins: design, characterization and activity. PeerJ 8, e9788.